Research Opportunities Database

Mark Johnson

Mark Johnson

Associate Professor of Biology

Department: Molecular Biology, Cell Biology, & Biochemistry

Phone: +1 401 863 3556

Phone 2: +1 401 863 3556

Email: Mark_Johnson_1@brown.edu

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Location:

Sidney Frank Hall, Room 160 - 863-3556

Research Summary

Molecular genetic analysis of plant reproductive development: Mechanisms of targeted pollen tube growth in Arabidopsis.

Past or Present Projects Available:

Identification of new genes required for pollen tube growth and guidance (experiments: molecular biology, PCR, gene cloning, gene expression of analysis); Characterization of pollen mutants that can't find their way to eggs (experiments: light microscopy, confocal microscopy, genetic crosses); Rapid evolution of genes mediating mating specificityin plants (experiments: DNA preparation from multiple plant species, PCR, gene sequencing, sequence comparisons); Investigation of hap 2 gene; Use of genetic engineering to determine whether HAPZ is a cellular fusogen; Glycobiology of pollen tube growth: investigating pollen tube elongation and guidance with a focus on proteins involved in carbohydrate biology; Functional study of beta-galactosidases 11 and 13 in arabidopsis thaliana pollen; Exploiting co-evolution in a computational screen for the discovery of HAP2 related genes; Identification of possible pathway partners for the Arabidopsis gene HAP2; A pair of beta-galactosidases is required for pollen tube penetration of the stigma; A study of beta-galactosidase 11 and beta-galactsidase 13'2 in pollen tube growth; How sweet is my pollen; A Mathematical Model of In-vivo Pollen Tube Growth; Identifying PP2A regulatory subunits that control ethylene production in Arabid; Beta-Galactosidases 11 and 13 and their Role in Arabidopsis Pollen tube Extension; Generating and Testing two Systems for labeling Pre-Synaptic Secretion of Neurotransmitters; The Function of BGAL in Pollen Tube Growth and Fertilizatiobn